• Login
    View Item 
    •   IUIU-IR Home
    • Faculty of Health Sciences
    • Research Papers
    • View Item
    •   IUIU-IR Home
    • Faculty of Health Sciences
    • Research Papers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR in Uganda with Quantitative Real Time RT-PCR on Field Samples

    Thumbnail
    View/Open
    1123-1-.pdf (685.4Kb)
    Date
    2016
    Author
    Mukasa, Hussein Kafeero
    Metadata
    Show full item record
    Abstract
    Foot-and-mouth disease (FMD) is a viral disease of Ungulates; both Artiodactyla and Perissodactyla. The mortality rates are low in adult animals but it affects milk yield and international trade. In endemic countries, diagnosis can be based on clinical signs. But these are shared by other vesicular diseases, so a laboratory is needed to confirm the disease. In Uganda the commonly used assays for the laboratory diagnosis of FMD are NSP ELISA and RT-PCR. Serology using ELISA techniques may fail to distinguish between vaccinated and new infection so compromising its sensitivity. The gel passed PCR is involves a lot of advance sample treatment increasing errors due to carry over which also compromises its sensitivity. This work reports comparative the detection of foot-and-mouth virus by NSP ELISA and RTPCR with real time PCR which was taken as the gold standard. The assays were compared in terms of sensitivity, specificity and disease prevalence and likelihood ratios. A total of 176 cattle were used from which samples that included epithelial tissues (17.05%) and oral swabs (84.09%) were collected from outbreak cases in Eastern Districts of Mbale and Budaka.
    URI
    http://hdl.handle.net/20.500.12309/495
    Collections
    • Research Papers [52]

    IUIU-IR copyright © 2018  Islamic University In Uganda
    Contact Us | Send Feedback

    Islamic University of Uganda
     

     

    Browse

    All of IUIU-IRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    IUIU-IR copyright © 2018  Islamic University In Uganda
    Contact Us | Send Feedback

    Islamic University of Uganda